Dr. S. L. Sasikala

Dr. S. L. Sasikala |Clyto Access

MSU, India

Keynote Speaker

Expertise:

Biography:

Dr. S. L. Sasikala graduated from the University of Madras. She initiated the process of signing Mevlana Protocol funded by Higher Education Council of Turkish Government for the Mobility of Faculty and Student exchange and Research Collaboration under Mevlana Programme with Agri Ibrahim Cecen University and Ataturk University, Erzurum, Turkey.  She trained around 800 teachers all over Kanyakumari Districton Disaster Management under Post Tsunami Livelihood Programme (PTSLP) which was implemented with the the assistance of IFAD from July 2009 to January 2010 and gave IFAD sponsored awareness program on various aspects of Science and Technology development, risks, hazards and management of natural disasters like tsunami to about 3500 coastal area school students in our center from May to July 2009. She presented 18 papers in international conferences and 51 papers in national conferences. She was awarded with “Young Scientist Project” by DST, New Delhi - Scheme for young Scientist. She is now Professor and Head, Center for marine science and technology, Manonmaniam Sundaranar University, Marina Campus, Tamil Nadu.

Presentation:

Title: Evaluation of DNA Damage Induced by heavy metal in the lobster Panulirus homarus Through Comet Assay

Abstract:

Toxicity of heavy metals is discussed in detail.  We are exposed to Aluminium through various sources such as drinking water, food and pharmaceutical products – medicines, antacids, vaccines, dialysis treatments etc.  Aluminium toxicity causes chronic renal failure, neurofibrillary degeneration, damage of cholinergic system. 

Aluminium chloride is a genotoxic agent causing DNA damage. In aquatic organisms DNA damage is associated with reduced growth and survival of embryos, larvae and adult. A study was carried out in the lobster, Panulirus homarus to assess early changes induced by aluminium exposure via direct route.  Apart from brain cells and reproductive cells concurrently, aluminium bioaccumulation was studied in the somatic tissues as well to understand the potential genotoxic biomarker responses.

Cell viability monitored prior to the lysis step using trypan blue showed substantial decrease in the viability of brain, ovarian, sperm and liver cells after aluminium exposure. The genotoxicity in the tissues of lobster exposed to aluminium chloride assessed through the extent of DNA damage using comet assay depicted little or no DNA damage in control while the damage was very obvious in aluminium exposed tissues.

Aluminium chloride was found to damage sperm cells and egg cells of the lobster H. americanus.  It is obvious that there is every possibilities  for such DNA damaged sperm cells and egg cells have very little chances of embryo formation nor even fertilization resulting in affected growth, reproduction and population dynamics. 

Related Conferences :

Marine Science Research and Technology Conference